Reactive oxygen species during plant-microorganism early interactions. “Influence of growth promoting bacteria from Uzbekistan and Germany on the growth and nutrient uptake of cotton and wheat on different soils,” in Plant Nutrition: Food Security and Sustainability of Agro-Ecosystems Through Basic and Applied Research Developments in plant and soil sciences, eds W. Horst, M. K. Schenk, A. Bürkert, N. Claassen, H. Flessa, W. B. Frommer, et al. Med. Plant Soil 321, 341–361. Mol. Because of the predominance of wild type cells along the roots, we also examined the composition of cells in the wash based on the assumption that this might represent loosely attached cells. In this case, we found a mixture of wild type and ΔcrtB mutant cells (Figure 6D), most of which appeared to be motile (data not shown). Natl. The genome of Pantoea sp. YR343 and related species within Enterobacteriaceae. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Fertil. Impact Factor 4.235 | CiteScore 6.4More on impact ›, Max Planck Institute for Plant Breeding Research, Germany, Austrian Institute of Technology (AIT), Austria, Ludwig Maximilian University of Munich, Germany. The decrease in Raman signal intensity was apparent even at H2O2 concentrations that did not result in decreased cell viability, consistent with the protective role of carotenoids in the presence of free oxygen radicals (Krinsky, 1989, 1993; Hirayama et al., 1994; Edge et al., 1997). YR343. Seedlings were incubated in a growth chamber at 24°C with a 12 h light and 12 h dark photoperiod. Pantoea sp. (F) Raman spectroscopy of wild type Pantoea sp. Carotenoids as modulators of lipid membrane physical properties. YR343 in LB, R2A, or M9 media, overnight cultures were diluted 1:100 into fresh medium and 200 μl was loaded into 12 wells on a honeycomb plate and placed into a Bioscreen C Reader System at room temperature with shaking overnight. How carotenoids function in photosynthetic bacteria. J. Photochem. Modulation of host immunity by beneficial microbes. We chose to further characterize this carotenoid using Raman spectroscopy. By this method, we observed a steady decrease in the spectral peak intensity corresponding to zeaxanthin (Figure 4F) which correlates to the loss of cell viability. Single bacterial colonies grown on R2A agar plates were transferred to 2 ml of sterile PBS, vortexed, and adjusted to OD600 = 0.2 using a Cary UV-Vis spectrophotometer. YR343 and ΔcrtB in M9 minimal medium. Mixtures of wild type and the ΔcrtB mutant were distinguished by colony color (wild type colonies were yellow, while the mutant colonies were white). (C) Colony morphology of Pantoea sp. There are several possible explanations for the role carotenoids may have in plant association. YR343 produced colonies that were irregularly shaped, wrinkly, and light yellow in color when grown on R2A agar plates (Figure 2C). The image on top shows roots from an uninoculated plant, while the bottom image shows roots colonized by YR343-pGFP. (1991). Biophys. Diversity of carotenoid synthesis gene clusters from environmental Enterobactetiaceae strains. Denitrification within the genus Azospirillum and other associative bacteria. FEMS Microbiol. doi: 10.1007/s001140050592. doi: 10.1371/journal.pone.0076382, Somers, E., Vanderleyden, J., and Srinivasan, M. (2004). La poursuite d’études des bacheliers professionnels (animateur : Emmanuel Quenson, Université d’Évry, Centre Pierre Naville)- Claire Lemêtre (Université Paris 8, CIRCEFT-ESCOL), Juliette Mengneau (Université de Nantes, CENS) \u0026 Sophie Orange (Université de Nantes, CENS), « La fac, on me dit que c'est possible mais que c'est pas faisable ». Jil Benedetto. The laser radiation was delivered via a single mode optical fiber through a dichroic beam splitter into the microscope objective and focused to a diffraction limited spot size on the surface of the sample. Roots were harvested as described above for Populus and analyzed for CFUs per gram of root material. YR343 is shown by a zone of clearing surrounding the colony. Syst. (2012). For these studies, wheat was used as the host plant due to its hardiness and ease of growth. For example, P. stewartii and P. ananatis are responsible for Stewart’s bacterial wilt disease on corn, leaf blotch disease in Sudangrass, and center rot in onion (Azad et al., 2000; Walcott et al., 2002). Mol. Les portes entrouvertes du supérieur pour les bacheliers professionnels- Cédric Hugrée (CNRS, CRESPPA-CSU), Deux cohortes de bacheliers professionnels face à la massification des premiers cycles universitaires- James Masy (Université de Nantes, CREN), Les bacheliers professionnels dans l’enseignement supérieur (A) Comparison of biofilm formation between wild type Pantoea sp. Acta 352, 361–370. YR343 possesses two gene clusters with distinct organizations representing both classes of cellulose synthesis gene clusters. We then compared production of indolic compounds, including IAA, in Pantoea sp. YR343 based on genomic comparisons. Alqueres, S., Meneses, C., Rouws, L., Rothballer, M., Baldani, I., Schmid, M., et al. Microbiol. doi: 10.1094/PHYTO.2000.90.4.368. doi: 10.1094/Phyto.03.10.0097. 52, 195–204. Bottom, predicted carotenoid biosynthesis pathway in Pantoea sp. These peaks are reduced in the ΔcrtB mutant. YR343 grown in R2A medium prior to planting the newly rooted Poplar plant. General Microbiol. We also analyzed motility behavior in this organism using LB medium containing either 0.3% (swimming) or 0.6% (swarming) agar (Figure 2E). 37, 932–936. doi: 10.1146/, Kulkarni, G., Busset, N., Molinaro, A., Gargani, D., Chaintreuil, C., Silipo, A., et al. We then tested the protective role of this carotenoid under oxidative stress created by exposure to hydrogen peroxide. YR343 on Populus deltoides WV94 growth. YR343. Plant Dis. Pseudomonas fluorescens induces strain-dependent and strain-independent host plant responses in defense networks, primary metabolism, photosynthesis, and fitness. Soils 47, 729–743. Molecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. From this perspective, the decrease in IAA production by the ΔcrtB mutant may be a consequence of changes in membrane fluidity or organization.